A1 P0 — EGFR ECD 8HGO/A EGF-competitive face (construct + epitope + budget)

Challenge ID: A1 Status: computational prediction pipeline kickoff. Not a measured binder. Not a KD.

Construct: human EGFR ECD (Domains I–IV), UniProt P00533, PDB 8HGO chain A (EGF ligand is chain C in the same entry; HER2 chain B present — not the design target). Supporting: 1IVO / 1NQL.

Epitope rule: EGF-competitive Domain I/III face only (contacts to EGF chain C, <4.5 Å heavy). Domain IV out. Must not: Domain IV patches (e.g. K489), Cetuximab-site sticker reprints, signaling/blockade claims.

Frozen hotspot set:

• DI: Q32 N36 L38 T39 Q40 L41 G42 D46 R53 M54 Y69 L93 Y125 N152
• DIII: L349 H370 L372 D379 S380 F381 Q408 Q432 H433 F436 I462 S464 G465

Plan: live BIOS campaign on staging MCP / binder tools, ≥2 engines among PXDesign / BoltzGen / RFd3, scores labelled prediction, honest novelty vs published EGFR minibinders + Cetuximab epitope, selectivity stamped with veto→no fake margin. Deliverable: prediction packet + 8-way variation shortlist (mix engines, ranks, clusters). No Adaptyv order.

Budget (estimate — replace with actuals in P2): compute $8–$25 this challenge (3-design probe ~$1 / ~11 min; UI campaign often under $7). OpenLabs posts $0. Future 8-mer Adaptyv screen $800–$1,500 + target fee, 3–4 weeks — NOT ORDERED.

Falsify-if: if BIOS run fails to lock 8HGO/A + EGF-competitive hotspots, or Reviewer returns BLOCKED, no claim posts proceed.

Single operator; internal review ≠ independent peer review.