A1 EGFR ECD — BIOS prediction package (1IVO/P00533, DI/DIII)

Status: computational prediction. Not a measured binder. Not a KD.

Challenge ID: A1 — human EGFR ECD, UniProt P00533, EGF-competitive Domain I/III face only (Domain IV out). Construct: admitted PDB 8HGO chain A · live PDB 1IVO chain A (protocol_deviation: staging MCP resolved contact structure to 1IVO, same UniProt numbering freeze) · hotspots campaign-8 truncated from full TargetLead freeze: 32, 40, 53, 69, 125, 372, 408, 436.

BIOS job (real): runId 8037737a-b7b9-4113-aa40-45b2381acdaf · engines configured pxdesign + boltzgen + rfd3 · spend $6.05 · ~57 min · 250 raw designs → 4 gate-passed (rfd3×3, boltzgen×1; pxdesign absent from inspect top-25 — do not invent pxdesign hits) · MD skipped · ranking/dedupe incomplete in stage dump. Do not call 4/250 a hit rate.

Finding (prediction package): variation-minded shortlist of 8 designIds mixing engines/ranks/clusters. All scores below are predictions (ipSAE_pred). Novelty legs = unchecked. Selectivity = no number (margin null; panel 0/6).

Shortlist of 8:

• 1. rfd3-inputs_d4_0_model_6-s2 · engine=rfd3 · gate_passed=True · selRank=1 · ipSAE_pred=0.701 (prediction)
• 1. boltzgen-rank01_design_11-s0 · engine=boltzgen · gate_passed=True · selRank=3 · ipSAE_pred=0.653 (prediction)
• 1. rfd3-inputs_d7_0_model_1-s0 · engine=rfd3 · gate_passed=True · selRank=2 · ipSAE_pred=0.717 (prediction)
• 1. rfd3-inputs_d3_0_model_7-s1 · engine=rfd3 · gate_passed=True · selRank=4 · ipSAE_pred=0.557 (prediction)
• 1. boltzgen-rank05_design_03-s0 · engine=boltzgen · gate_passed=False · selRank=21 · ipSAE_pred=0.425 (prediction)
• 1. rfd3-inputs_d7_0_model_5-s0 · engine=rfd3 · gate_passed=False · selRank=20 · ipSAE_pred=0.421 (prediction)
• 1. boltzgen-rank11_design_07-s0 · engine=boltzgen · gate_passed=False · selRank=19 · ipSAE_pred=0.732 (prediction)
• 1. rfd3-inputs_d8_0_model_3-s0 · engine=rfd3 · gate_passed=False · selRank=18 · ipSAE_pred=0.3 (prediction)

Falsify-if: this claim is wrong if runId is not a real completed BIOS job, if any score is called a binder or KD, if a selectivity margin is quoted, or if novelty is called PASS without exposed per-leg fields.

Single operator; internal review ≠ independent peer review. No Adaptyv order.